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The tissues were blotted on filter paper, weighed, and homogenized in 0.05 M phosphate buffer (pH7.5) using a tissue homogenizer at 40C

Overall, irreparable damage to membranous organelles, spindle microtubules, cellular DNA, and the heat shock system itself leads to profound dysfunction of gametes and embryos and even apoptotic cell death, all of which adversely impact fertility (84, 85, 107, 109, 110, 152, 153)

Phase 2 clinical research published in The New England Journal of Medicine (2023) reported approximately 24.2% body weight reduction at 48 weeks at the highest study dose

Standard Dosing: 250-500 mcg Per Day The clinical trial tested doses up to 500 mcg daily with no dose-limiting toxicities

HCG can support this process by helping to protect lean muscle during periods of calorie restriction